人感染H7N9禽流感病毒核酸檢測試劑盒

廣州健侖生物科技有限公司

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人感染H7N9禽流感病毒核酸檢測試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

實驗步驟
1．首先將內層滅菌桶取出，再向外層鍋內加入適量的水，使水面與三角擱架相平為宜。
2．放回滅菌桶，并裝入待滅菌物品。注意不要裝得太擠，以免防礙蒸汽流通而影響滅菌效果。三角燒瓶與試管口端均不要與桶壁接觸，以免冷凝水淋濕包口的紙而透入棉塞。
3．加蓋，并將蓋上的排氣軟管插入內層滅菌桶的排氣槽內。再以兩兩對稱的方式同時旋緊相對的兩個螺栓，使螺栓松緊*，勿使漏氣。
4．用電爐或煤氣加熱，并同時打開排氣閥，使水沸騰以排除鍋內的冷空氣。待冷空氣*排盡后，關上排氣閥，讓鍋內的溫度隨蒸汽壓力增加而逐漸上升。當鍋內壓力升到所需壓力時，控制熱源，維持壓力至所需時間。本實驗用 1．05kg/cm2，121．3℃，20分鐘滅菌。
5．滅菌所需時間到后，切斷電源或關閉煤氣，讓滅菌鍋內溫度自然下降，當壓力表的壓力降至0時，打開排氣閥，旋松螺栓，打開蓋子，取出滅菌物品。如果壓力未降到0時，打開排氣閥，就會因鍋內壓力突然下降，使容器內的培養基由于內外壓力不平衡而沖出燒瓶口或試管口，造成棉塞沾染培養基而發生污染。
6．將取出的滅菌培養基放入 37℃溫箱培養24小時，經檢查若無雜菌生長，即可待

Experimental steps
1. First, remove the inner sterilization barrel, and then add an appropriate amount of water to the outer pot so that the water level with the triangular shelf is appropriate.
2. Replace the sterilizer and load the items to be sterilized. Be careful not to install too crowded, so as not to hinder the flow of steam and affect the sterilization effect. Conical flask and test tube mouth end are not in contact with the barrel wall, so as not to condensate wet wet tipping paper into the tampon.
3. Cap and insert the capped vent hose into the vent tank in the inner sterilizer. Tighten the two bolts at the same time in two symmetrical ways, so that the bolts are tight and tight, and do not leak.
4. Use electric furnace or gas heating, and at the same time open the exhaust valve, the water boiling to exclude cold air inside the pot. After the cold air is compley drained off, close the exhaust valve so that the temperature inside the pan gradually increases with the increase of steam pressure. When the pressure in the pan rises to the desired pressure, the heat source is controlled and the pressure is maintained until the desired time. The experiment with 1.05kg / cm2, 121.3 ℃, 20 minutes sterilization.
5. Turn off the power or turn off the gas to allow the temperature in the autoclave to drop naturally. When the gauge pressure drops to 0, open the vent valve, loosen the screw, open the lid and remove the sterilized items. If the pressure has not dropped to 0, open the exhaust valve, the pressure will be due to a sudden drop in the pot, so that the media inside the container due to internal and external pressure imbalance and out of the flask mouth or test tube mouth, resulting in tampon contaminated medium occurred Pollution.
6. Will be taken out of sterile medium incubated in 37 ℃ incubator 24 hours, after checking if no bacteria growth, you can wait