PCR試劑 流感甲乙型/合胞病毒AB型四重檢測試劑盒

廣州健侖生物科技有限公司

Ready to use lyo master mix (8-well strips each) for detection of influenza A virus, influenza B virus and human respiratory syncytial viruses A and B including internal control.
準備使用lyo master混合物（每個8孔條）檢測甲型流感病毒，乙型流感病毒和人類呼吸道合胞病毒A和B（包括內部對照）。
Principle
Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology
Targets
Lyo mastermix:
influenza A virus
influenza B virus
human respiratory syncytial virus A/B
internal Control
Specimen
This test is for use with extracted nucleic acid from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin
Storage
Liquid and lyophilised components: 2-8°C until expiration date
Sealing foils: room temperature
Shelf life
12 months from manufacture

PCR試劑 流感甲乙型/合胞病毒AB型四重檢測試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

【】 
【騰訊  】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

1．  固定：用4%的多聚甲醛固定液。對于冰凍切片，甲醛固定有時比冰凍丙酮好；但對于不同的組織和抗原，可選用不同的固定液。
Bouin S固定液：飽和苦味酸750ml，甲醛250ml，冰醋酸50ml，其對組織的穿透力較強，固定較好，結構完整，但因偏酸，對抗原有一定損害，且組織收縮明顯，不適于組織標本的長期保存。 
PLP液：即高碘酸鈉-賴氨酸-多聚甲醛，適于固定石蠟切片。適于富含糖類組織，對超微結構及許多抗原的抗原性保存較好。 
2．組織脫水，透明：時間不能太長，否則在切片時容易碎片，切不完整。 
3．切片時展片：有些組織在切片后難以在水中展開，這時可適當地在水中加入幾滴乙醇。 
4．烤片：60℃ 30分鐘或37℃ 過夜，溫度太高或時間太長，抗原容易丟失。 
5．蠟塊及切片的保存：在4℃保存 
6．脫片問題： Poly-L-Lysine(多聚賴氨酸)為目前免疫組化染色工作中zui常用的一種防脫片劑，6ml的多聚賴氨酸溶液可按1:10稀釋成60ml的工作液，適合于需要酶消化、微波、高溫高壓的防脫片處理。如不行，可用雙重處理（APES和Poly-L-Lysine）的切片。在以上兩種條件都行不通的情況下，可用如下方法：切片在脫蠟前，放在APES 1：50 丙酮溶液中浸泡3分鐘，晾干，即可進行下一步。 
7．滅活內源性酶：HRP系統：3%雙氧水滅活；AP系統：3%HAc滅活。 
8．暴露抗原：對于石蠟切片的免疫組化實驗時，必須采用高溫加熱抗原修復，這將有助于暴露抗原決定簇，從而增加免疫組化染色的強度(不同抗體的*修復液請參閱抗體說明書)。對于不同的組織，不同的抗原，不同的抗體，所采用的方法應不一樣，可進行熱修復、胰酶消化、既不修復也不消化。膠原還可以用胃蛋白酶消化等。 

1. Fixed: It is best to use 4% paraformaldehyde fixative. For frozen sections, formaldehyde fixation is sometimes better than frozen acetone; however, different fixatives may be used for different tissues and antigens.
Bouin S fixed solution: saturated picric acid 750ml, formaldehyde 250ml, glacial acetic acid 50ml, the penetration of its stronger, better fixed, structural integrity, but due to acid, have some damage to the antigen, and tissue shrink significantly, Not suitable for long-term preservation of tissue specimens.
PLP solution: sodium periodate - lysine - paraformaldehyde, suitable for fixed paraffin sections. Suitable for rich in carbohydrate tissue, the antigenicity of the ultrastructure and many antigens is better preserved.
2. Tissue dehydration, transparent: the time can not be too long, otherwise easy to fragment in the slice, cut incomplete.
3. Splinting: Some tissues are difficult to unfold in water after slicing, when a few drops of ethanol are properly added to the water.
4. Baked pieces: 60 ℃ 30 minutes or 37 ℃ overnight, the temperature is too high or too long, the antigen is easy to lose.
5. Preservation of wax blocks and slices: best preserved at 4 ℃
6. The problem of delamination: Poly-L-Lysine (Polylysine) is the most commonly used anti-drop tablet for immunohistochemical staining. 6ml of polylysine solution can be diluted 1:10 into 60ml The working fluid, suitable for digestion, microwave, high temperature and pressure of the anti-off film processing. If not, double-stained sections (APES and Poly-L-Lysine) can be used. In the case of the above two conditions are not feasible, the following method can be used: slice before dewaxing, put in APES 1:50 acetone solution soak for 3 minutes, dry, you can proceed to the next step.
7. Inactivation of endogenous enzymes: HRP system: 3% hydrogen peroxide inactivated; AP system: 3% HAc inactivated.
8. Exposure to Antigens: For immunohistochemistry of paraffin sections, high-temperature heating of the antigen must be used to repair the antigen, which will help to expose the antigenic determinants and thereby increase the intensity of immunohistochemical staining (see Antibody manual for optimal antibody repair of different antibodies ). For different tissues, different antigens, different antibodies, the method used should be different, can be heat repair, trypsin digestion, neither repair nor digestion. Collagen can also be digested with pepsin and so on.