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【公司名稱】廣州健侖生物科技有限公司

【市場部】楊永漢
【】
【騰訊】
【公司地址】廣州市清華科技園健新基地番禺石樓鎮(zhèn)健啟路63號二期2幢101-103室

分別處移動(dòng)圖片理肺泡巨噬細(xì)胞和肺組織中的巨噬細(xì)胞：離心200×g10分鐘，去上清液。輕彈試管，懸浮細(xì)胞，加入10ml低滲液（20mmol/LTris,0.75%氯化銨，pH7.4），37℃處理3～5分鐘，溶解紅細(xì)胞。紅細(xì)胞通常分別占肺泡巨噬細(xì)胞和組織巨噬細(xì)胞的 1%和10%。也可以不用低滲處理，直接在淋巴細(xì)胞分離液上分離巨噬細(xì)胞。5、離心200×g10分鐘，去上清液。用RPMI-1640培養(yǎng)液洗滌細(xì)胞一次。將細(xì)胞懸液加在淋巴細(xì)胞分離液（比重1.077）上，離心400×g20分鐘，收集交界面的巨噬細(xì)胞。棄去沉淀的死細(xì) 胞和紅細(xì)胞。6、用RPMI-1640培養(yǎng)液洗滌巨噬細(xì)胞2次。臺盼藍(lán)染色檢測細(xì)胞活力和細(xì)胞數(shù)，將細(xì)胞配成所需濃度。此時(shí)巨噬細(xì)胞活力可達(dá)90%以上，巨噬細(xì)胞占全部細(xì)胞的90%以上巨噬細(xì)胞游走抑制因子(macrophage migration inhibitory factor，MIF) 是集細(xì)胞因子、生長因子、激素和酶特性于一身的多效能蛋白分子。MIF高度保守，在多種急慢性炎癥性疾病中發(fā)揮多種免疫功能。MIF參與動(dòng)脈粥樣硬化發(fā)病和發(fā)展，即斑塊形成、動(dòng)脈損傷后重建、動(dòng)脈瘤形成、心肌梗死、缺血再灌注損傷等機(jī)制。此外，其他形式的心肌功能不全和炎癥與MIF參與血管再生有關(guān)。
Displace the macrophage in the lung alveolar macrophages and lung tissues by moving them: Centrifuge at 200×g for 10 minutes and remove the supernatant. Gently test the tube, suspend the cells, add 10 ml of hypotonic solution (20 mmol/LTris, 0.75% ammonium chloride, pH 7.4), and treat at 37°C for 3 to 5 minutes to dissolve red blood cells. Erythrocytes usually account for 1% and 10% of alveolar macrophages and tissue macrophages, respectively. It is also possible to isolate macrophages directly on lymphocyte separations without hypotonic treatment. 5. Centrifuge at 200×g for 10 minutes and remove the supernatant. Wash the cells once with RPMI-1640 medium. The cell suspension was added to a lymphocyte separation solution (specific gravity: 1.077) and centrifuged at 400×g for 20 minutes to collect macrophages at the interface. The precipitated dead and red blood cells are discarded. 6. Wash macrophages 2 times with RPMI-1640 medium. Trypan blue dye was used to measure cell viability and cell number, and the cells were dubbed to the desired concentration. At this time, macrophage viability can reach more than 90%, and macrophages account for more than 90% of all cells. Macrophage migration inhibitory factor (MIF) is a cytokine, growth factor, hormone and enzyme characteristic. A multi-functioning protein molecule. MIF is highly conserved and exerts multiple immune functions in a variety of acute and chronic inflammatory diseases. MIF is involved in the pathogenesis and development of atherosclerosis, namely plaque formation, remodeling after arterial injury, aneurysm formation, myocardial infarction, ischemia-reperfusion injury and other mechanisms. In addition, other forms of myocardial insufficiency and inflammation are associated with MIF involvement in angiogenesis.

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